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In vitro transcription of the glyQS and tyrS leader regions. Lanes 1–5, glyQS DNA; lanes 6–11, tyrS DNA. Lanes 1, 3, and 6, no tRNA added; lanes 2, 4, and 7, tRNAGly (T7 transcript); lanes 5, 8, and 11, tRNATyr (T7 transcript); lane 9, E. coli tRNATyr (modified, purchased from Sigma); lanes 3–9, NusA added; lanes 1, 2, 10, and 11, no NusA. T, terminated transcript; RT, read-through transcript. Percent read-through is indicated at the bottom of each lane.
