Fig 3.
Ensemble wild-type and hairpin 2 mutant kinetics as seen from different reaction coordinates. (A) W43 burial. The end of the burst phase (labeled as “burst phase”) was determined by matching the wild-type W43 burial with the burst phase value reported in ref. 25. The wild-type and mutant post-burst-phase data were fitted with single exponential rates of 2.56 × 10−9 and 9.51 × 10−10, respectively. (B) Backbone drms, and secondary structure details. The wild-type ensemble settles to low drms faster than the mutant ensemble (Top). Under ensemble averaging, the wild-type hairpin 2 appears to form earlier than the other three secondary structure features (Middle), whereas this sequence is reversed in the mutant (Bottom).