Fig 3.

Histological and in situ analyses of the transgenic mammary gland. (A–C) Sections were stained with hematoxylin and eosin (H&E). Comparison of controls (Ctrl: monogenics or wild types treated with RU486 or bigenics treated with placebo in A) and bigenics (Bi-Tg) given RU486 for 2 months showed that the bigenic glandular epithelium was multilayered and loosely associated (B) in contrast to a uniform singular layer of cells in the control (A). In a more hyperplastic region (C), the bigenic epithelium was completely disorganized and surrounded by dense connective tissue. (D–F) By in situ hybridization (ISH), high levels of FGF-3 expression were detected as blue-black precipitates in the bigenic (E, F) but not in control epithelium of monogenic mice given 2 months of RU486 treatment (D).