Fig 2.

SOX9 contains a CRM1-dependent NES. COS-7 (A) and NIH 3T3 (B) cells were transiently transfected with a SOX9 expression vector and treated with (2.5 ng/ml) LMB when indicated (+LMB). Cells were costained with SOX9 Ab (Left) and Hoechst dye (HST, Right). (A) In COS-7 cells, SOX9 was strictly nuclear and was not affected by LMB (not shown). (B) Conversely in NIH 3T3 cells, SOX9 is also cytoplasmic (−LMB). This localization is the result of nuclear export because after inhibition by LMB all SOX9 remains in the nucleus (+LMB).