TABLE 1.
NAAT performances for MTBC detection vs culture as reference standard according to the type of samples (n = 685)
| Type of sample | Number of samples |
NAAT+ /C+ |
NAAT+ /C− |
NAAT- /C+ |
NAAT- /C− |
Sensitivity % (CI 95%) |
Specificity % (CI 95%) |
Agreement % (CI 95%) |
|---|---|---|---|---|---|---|---|---|
| Digestive | 36 | 2 | 1 | 0 | 33 | 100 (15.8–100) |
97.1 (84.7–99.9) |
97.2 (85.8–99.5) |
| Lymph nodes | 285 | 19 | 11 | 3 | 252 | 86.4 (65.1–97.1) |
95.8 (92.6–97.9) |
95.1 (91.9–97.0) |
| Bones and joint | 113 | 17 | 7 | 1 | 88 | 94.4 (83.8–100) |
92.6 (85.3–96.9) |
92.9 (86.6–96.4) |
| Serous fluid total | 194 | 3 | 10 | 2 | 179 | 60.0 (14.6–94.7) |
94.7 (90.5–97.4) |
93.8 (89.5–96.4) |
| Cerebrospinal fluid | 73 | 0 | 7 | 0 | 66 | N/A | 90.4 (81.2–96.1) |
N/A |
| Other serous fluid | 13 | 1 | 2 | 0 | 10 | 100 (2.5–100) |
83.3 (51.6–97.9) |
N/A |
| Ascites fluid | 20 | 1 | 0 | 0 | 19 | 100 (2.5–100) |
100 (84.2–100) |
N/A |
| Pleural fluid | 82 | 1 | 1 | 2 | 78 | 33.3 (0.84–90.5) |
98.7 (93.1–99.9) |
N/A |
| Peritoneal fluid | 6 | 0 | 0 | 0 | 6 | N/A | 100 (54.1–100) |
N/A |
| Pus | 57 | 10 | 5 | 1 | 41 | 90.9 (58.7–99.8) |
89.1 (76.4–96.4) |
89.5 (78.9–95.0) |
| Total | 685a | 51 | 34 | 7 | 593 | 87.9 (76.7–95.0) |
94.6 (92.5–96.2) |
94.0 (92.0–95.6) |
a
Exclusion of invalid tests; C+: MTBC positive culture; C−: MTBC negative culture; NAAT+: Positive ULTRA; NAAT−: Negative ULTRA ; N/A: non-applicable.