Figure 2 –

Physiological and metabolic adaptions to PoWeR. (A) Average daily voluntary running distance per week during 8-weeks of PoWeR. (B) Cumulative and average daily running distance over the duration of PoWeR per mouse. (C) Bodyweight, (D) fat free mass, and (E) fat mass body composition measured by EchoMRI. (F) Muscle mass of soleus (SOL), plantaris (PLA), flexor digitorum longus (FDL), tibialis anterior (TA), and quadriceps (QUAD) expressed relative to Sed. (G) Heart and (H) Liver mass normalized to body mass and expressed relative to sedentary. (I) Time run during a maximal graded exercise treadmill test. (J) Grip strength expressed as absolute grams of force. (K) Glucose tolerance test and (L) Pyruvate tolerance test with respective area of the curve. (M) Femoral bone mineral density measured by μCT. N=9–10 per group. Mean plus individual data points or error bars represent mean ± SEM. 2-way ANOVA with repeated measures was used to assess main effects of group, time, and group x time interaction (B, J, K, L) otherwise unpaired t-test (C-E, F-I, M). *P<0.05, **P<0.01, ***P<0.001.