Fig 2.

Isoelectric focusing and activity of purified GSS. (A) Isoelectric focusing of 10 μg natively purified DBM-GSS at pH 10 to 3, double stained with Bio-Safe Coomassie G250 and silver (Bio-Rad). (B) A further lane from the isoelectric focusing gel, loaded with 10 μg natively purified GSS, was divided vertically into 28 parts of 2 mm length. Gel pieces were suspended in 100 μl of 100 mM Tris⋅HCl, pH 7.5, and 30 μl each were tested for GSS activity. Vertical axis shows relative amounts of sinigrin converted to its desulfo-form, as measured by HPLC. Maximum GSS activity was detected in fraction 15.