Fig 5.

Immunoblots localize DBM GSS to larval guts. (Upper) SDS/PAGE protein gel. Molecular mass is indicated on the left. Lane 1, 5 μg body without gut; lane 2, 5 μg gut tissue; lane 3, 3 μg gut content; lane 4, 0.1 μg native PAGE-purified DBM GSS (indicated by an arrow); lane 5, 1 μg Nickel-agarose purified GSS, heterologously expressed by E. coli; lane 6, 5 μg E. coli total protein with control vector without GSS insert; lane 7, 5 μg Helix pomatia sulfatase, crude extract, type H-1 (Sigma). (Lower) Immunoblot detection with polyclonal GSS antibody including a second anti-rabbit antibody. For immunoblotting one-tenth of the protein amounts from Upper was loaded, except for lane 4, where one-half was used. Adjustment of protein amounts was carried out to optimize staining.