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RT-PCR for amplification of the HA and NA segments. RT-PCR was performed with the primer pair Bm-NAb-1/Bm-NAb-1557R. The PCR products were subjected to gel electrophoresis on a 1% agarose gel. RNA isolated from the following virus isolates were used: 1, B/Lee/40; 2, B/Ann Arbor/1/94; 3, B/Yamanashi/166/98; 4, B/Johannesburg/5/99; 5, B/Victoria/504/2000; 6, B/Sichuan/317/2001; 7, B/Shizuoka/15/2001; 8, B/Hawaii/10/2001; 9, B/Hong Kong/330/2001; –, no RNA.
