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. 2002 Aug 2;99(17):11441–11446. doi: 10.1073/pnas.172254699

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Copyright © 2002, The National Academy of Sciences

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Fig 2. — Determination of subunit stoichiometry from the single-channel current amplitude. Coexpression of TEA insensitive (Y334V) and Ca-bowl mutated (ΔD965–ΔD966) subunits yields BK channels whose current amplitudes fall into one of four levels in the presence of 1.5 mM TEA_O. (a–d) Representative single-channel recordings and all-points amplitude histograms for the four levels of observed currents. The stoichiometry for each of these channels, as deduced from the single-channel current amplitudes, is shown at the right. (e) A one-dimensional scatter plot of the current amplitude recorded from each of 18 channels shows clustering into four distinct current levels. Ca adjusted for a–d to obtain a high Po. Horizontal bar, 100 ms; vertical bar, 5 pA. +50 mV.

Inline graphic — Determination of subunit stoichiometry from the single-channel current amplitude. Coexpression of TEA insensitive (Y334V) and Ca-bowl mutated (ΔD965–ΔD966) subunits yields BK channels whose current amplitudes fall into one of four levels in the presence of 1.5 mM TEA_O. (a–d) Representative single-channel recordings and all-points amplitude histograms for the four levels of observed currents. The stoichiometry for each of these channels, as deduced from the single-channel current amplitudes, is shown at the right. (e) A one-dimensional scatter plot of the current amplitude recorded from each of 18 channels shows clustering into four distinct current levels. Ca adjusted for a–d to obtain a high Po. Horizontal bar, 100 ms; vertical bar, 5 pA. +50 mV.