Figure 5.

PADMA 28 interferes with Bcl-2 function. CEM-C7H2 as well as CEM-C7H2Bcl2-TET-off cells were cultivated in the presence (Bcl2 repressed) or absence (Bcl2 expressed) of 200 μg/ml tetracycline; 24 h after seeding, cells were treated with a solution of 0.4 mg/ml PADMA 28 (1:200 EtOH). After 24 and 48 h, cells were harvested and analysed by FACS as described under Methods. Data are expressed as percent of untreated solvent controls (± SEM, ± SEM, p < 0.03) of at least three independent experiments done in triplicate.