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[Preprint]. 2025 Jul 22:2025.07.18.665589. [Version 1] doi: 10.1101/2025.07.18.665589

Figure 1. Craniofacial phenotypes in her9 mutant zebrafish.

Figure 1.

A-B) Gross morphology of her9 mutant larvae at 5 dpf compared with WT and heterozygous larvae. Her9 mutants lack a swim bladder (SB, arrow) and have abnormally shaped jaws (arrowheads). C) Absence of swim bladder is a fully penetrant phenotype in her9 mutants. D) Schematic of zebrafish craniofacial structures. E-G’) Variations in her9 mutant craniofacial defects visualized with Alcian blue staining of cartilage. H) Frequency of craniofacial defects observed in her9 vs. WT and heterozygous larvae. I-J) Measurement of distance between the eyes of WT and her9 mutant larvae. Hs, hyosymplectic; bh, basihyal; ch, ceratohyal; m, Meckel’s cartilage; pq, palatoquadrate; cb, ceratobranchial arches; hb, hinge bone; tr, trabeculae; ih, interhyal; e, ethmoid plate. Scale bars = 25 μm.