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[Preprint]. 2025 Jul 22:2025.07.19.665705. [Version 1] doi: 10.1101/2025.07.19.665705

Figure 4. IFT cardiomyocyte number is relatively normal in smo;laf double mutants.

Figure 4.

(A,B) In situ hybridization depicts bmp4 expression at 48 hpf. Frontal views; arrowheads indicate the IFT. bmp4 expression is restored to a relatively normal pattern and intensity in the IFT of smo;laf double mutants (B, n=8), comparable to wt siblings (A, n=5). Scale bars: 50 μm.

(C-F) Immunofluorescence depicts Isl1 (white) localization in the myocardium (MF20, green) at 32 hpf. This timepoint was chosen because smo;laf embryos reliably survive to 32 hpf, whereas they have variable survival at later stages. Lateral views; arrowheads indicate the IFT. Note that the arterial pole is less visible in these images than elsewhere due to the position of the heart at 32 hpf.

(G) Graph indicates the number of Isl1+ cardiomyocytes in the IFT at 32 hpf. Isl1+ IFT cardiomyocyte number is increased in smo (E), decreased in laf (D), and relatively normal in smo;laf (F), relative to wt siblings (C). ***p<0.001.