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[Preprint]. 2025 Jul 22:2025.07.19.665705. [Version 1] doi: 10.1101/2025.07.19.665705

Figure 5. IFT cardiomyocyte number is reduced in laf mutants by 24 hpf.

Figure 5.

(A,B,E,F) Immunofluorescence in Tg(myl7:H2A-mCherry) embryos depicts mCherry (green) in cardiomyocyte nuclei, as well as Amhc (magenta) and Isl1 (white) localization, in laf mutant embryos (B,F) and wt siblings (A,E) at 48 (A,B) or 24 (E,F) hpf. (A’,B’,E’,F’) Isl1 localization is shown in white. Frontal views, arrowheads indicate the IFT. At both stages, laf mutants exhibit fewer Isl1+ IFT cardiomyocytes than their wt siblings. Scale bars: 30 μm.

(C,G) Graphs indicate the number of Isl1+ IFT cardiomyocytes in laf mutants and wt siblings at 48 (C) and 24 (G) hpf. The number of Isl1+ IFT cells is significantly reduced in laf mutants at both stages. ****p<0.0001.

(D,H) Graphs indicate the number of Amhc+ Isl1− atrial cardiomyocytes and Amhc− Isl1− ventricular cardiomyocytes in laf mutants and wt siblings at 48 (D) and 24 (H) hpf. See Materials and Methods for cell counting technique. At both stages, laf mutants have fewer atrial cardiomyocytes than their wt siblings. *p<0.05; **p<0.01; ****p<0.0001.