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[Preprint]. 2025 Jul 22:2025.07.19.665705. [Version 1] doi: 10.1101/2025.07.19.665705

Figure 6. Bmp signaling acts during gastrulation to promote IFT cardiomyocyte production.

Figure 6.

(A-D) Immunofluorescence in Tg(myl7:H2A-mCherry) embryos at 48 hpf (as in Fig. 5A,B) shows effects of treatment with 1% DMSO (A), DMH1 from 40% epiboly (B), DMH1 from tailbud (C), and DMH1 from 24 hpf (D). (A’,B’,C’,D’) Isl1 localization is shown in white. Frontal views, arrowheads indicate the IFT. Treatment with DMH1 beginning at 40% epiboly reduces the number of Isl1+ IFT cardiomyocytes, reminiscent of the reduction seen in laf mutants (Fig. 5B,C), whereas treatment with DMH1 beginning at tailbud or 24 hpf does not seem to disrupt the formation of IFT cells. Scale bars: 30 μm.

(E) Graph indicates the number of Isl1+ IFT cardiomyocytes in embryos treated with DMSO or with DMH1 at 40% epiboly, tailbud, or 24 hpf. ***p<0.001.

(F) Graph indicates the numbers of Amhc+ Isl1− atrial cardiomyocytes and Amhc− Isl1− ventricular cardiomyocytes at 48 hpf in DMSO-treated controls and in embryos treated with DMH1 beginning at 40% epiboly. **p<0.01.