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[Preprint]. 2025 Jul 22:2025.07.19.665705. [Version 1] doi: 10.1101/2025.07.19.665705

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Figure 7. — (A,B,E,F) Immunofluorescence in Tg(myl7:H2A-mCherry) embryos depicts mCherry (green) in cardiomyocyte nuclei, as well as Amhc (magenta) and Isl1 (white) localization, in chd mutant embryos (B,F) and wt siblings (A,E) at 48 (A,B) or 24 (E,F) hpf. (A’,B’,E’,F’) Isl1 localization is shown in white. Frontal views, arrowheads indicate the IFT. At both stages, chd mutants exhibit more Isl1+ IFT cardiomyocytes than their wt siblings. Scale bars: 30 μm.

(C,G) Graphs indicate the number of Isl1+ IFT cardiomyocytes in chd mutants and wt siblings at 48 (C) and 24 (G) hpf. The number of Isl1+ IFT cells is significantly increased in chd mutants at both stages. ****p<0.0001.

(D,H) Graphs indicate the number of Amhc+ Isl1− atrial cardiomyocytes and Amhc− Isl1− ventricular cardiomyocytes in chd mutants and wt siblings at 48 (D) and 24 (H) hpf. At both stages, chd mutants have fewer Amhc+ Isl1− atrial cardiomyocytes than their wt siblings. Additionally, chd mutants have fewer ventricular cardiomyocytes, compared to their wt siblings, at both stages. ***p<0.001; ****p<0.0001.