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[Preprint]. 2025 Jul 22:2025.07.19.665705. [Version 1] doi: 10.1101/2025.07.19.665705

Figure 8. Inhibition of Wnt signaling limits IFT expansion in chd mutants, but not in smo mutants.

Figure 8.

(A-H) Immunofluorescence at 48 hpf depicts the effects of treatment with DMSO (A,C,E,G) or PNU-74654 (B,D,F,H) beginning at 16 s in smo mutants (C,D), chd mutants (G,H), and wt siblings (A,B,E,F). Isl1 (white) marks the IFT cardiomyocytes; MF20 marks the myocardium (green). Frontal views; arrowheads indicate the IFT. Scale bars: 30 μm.

(I,J) Graphs indicate the effects of DMSO or PNU-74654 treatment on the number of Isl1+ IFT cardiomyocytes at 48 hpf in smo mutants (I) and chd mutants (J), with comparisons to wt siblings. Inhibition of Wnt signaling reduces the number of Isl1+ IFT cardiomyocytes in wt embryos (I,J) and in chd mutants (J). In contrast, smo mutants exhibit an increased number of Isl1+ IFT cardiomyocytes even after treatment with PNU-74654 (I). **p<0.01; ***p<0.001; ****p<0.0001.