Figure 7. Tracking the progenitors of spineless-expressing cells in the distal carpus.

Snapshots of live recording of a regenerating Parhyale T5 leg at 0, 18, 60, 76, 85, and 97 h post amputation (left) and corresponding illustrations of the same legs (right). Coloured circles highlight cells that contribute to spineless-expressing cells in the distal part of the carpus; each colour highlights the lineage of a distinct progenitor cell. In the bottom-right panel, spineless-expressing nuclei (identified by HCR) are marked by filled circles, whereas spineless-non-expressing nuclei derived from the same progenitors are marked by open circles. The images show single optical sections as they appear in the Mastodon user interface; nuclei that are only partly captured in the current optical section appear as smaller circles. Distal parts of the leg oriented towards the right.

Figure 7—figure supplement 1. Spineless expression in a regenerating Parhyale leg.

(Top) T5 Parhyale leg at 97 h post amputation (same leg as in Figure 7) stained with HCR probes for spineless (in green) and futsch (in red), and with DAPI (in blue). Nascent transcripts of spineless are visible in a band of cells at the distal end of the carpus (arrowhead), and in other cells in the carpus, propodus, and dactylus. Autofluorescence is visible in the cuticle (in green) and in granular cells (in both red and green channels, appearing yellow). No futsch-stained neurons are visible in this optical slice. Scale bar, 20 µm. (Bottom) Topology of the lineage trees generating the tracked spineless-positive cells, and number of progenitors tracked with each tree topology.