Table 2.
PCR cycling conditions.
| Stage | Temperature (°C) | Time (min) | Cycles |
|---|---|---|---|
| Initial denaturation | 95 | 5 | 1 |
|
| |||
| Denaturation | 95 | 1 | 40 |
| Annealing | 50 | 1 | |
| Elongation | 72 | 2 | |
|
| |||
| Final extension | 72 | 7 | 1 |
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