Fig. 5.

In vivo imaging of vasculatures in mouse hindlimb leg and chicken embryo’s CAM. (a) A 5-week-old nude mouse placed in the sample arm of the all-optical PAT system for in vivo imaging through the intact skin. (b) A photograph of the blood vessels in the hindlimb, taken after imaging session, which was used as ground truth. (c) A photograph taken after further removing the fat tissue revealing vascular structures underneath (area 1). Two vessels of artery and vein are distinctly visible in area 2. (d) The 3D image of the mouse hindlimb reconstructed with the multilayer backpropagation method. Scale bars in (b)-(d): 2 mm. (e) A photograph and its enlarged image of the blood vessels formed in the CAM of chicken embryo incubated for 11 days. Scale bar: 1 mm. (f) Reconstructed volumetric vasculature image of the CAM. Scale bar: 2 mm.