Figure 4. No changes in the q of TC→ET synapses after NE.

(A, C) Average amplitude of Sr²⁺-mEPSCs in ETs in response to L5–6 thalamocortical maximal photostimulation in 1d (A) and 7d (B), post SE (black) and NE (red) (1d SE: 5 cells/4 mice; 1d NE: 7 cells/5 mice; 7d SE: 7 cells/2 mice; 7d NE: 5 cells/2 mice).

(B, D) Representative Sr²⁺-mEPSCs traces (top left) and representative average traces of quantal Sr²⁺-mEPSCs traces (top right). Amplitude histogram of events before (background noise) and after stimulation from the same cell (bottom) in 1d (B) and 7d (D) after SE (black) and NE (red) mice. The arrowhead indicates the onset of light stimulus. The dotted line indicates 400ms time window before stimulus (pre-LED). The solid line represents a 400ms time window, which started 100 ms after the stimulus (post-LED) and was used to analyze the amplitude of Sr²⁺-mEPSCs (1d SE: 5 cells/4 mice; 1d NE: 7 cells/5 mice; 7d SE: 7 cells/2 mice; 7d NE: 5 cells/2 mice).

(E) Representative traces of L5 ET EPSCs in response to L5–6 thalamocortical maximal photostimulation in in 1d SE (E₁, black) vs. 1d NE (E₁, red), 7d SE (E₂, black) vs. 7d NE (E₂, red) mice.

(F) Summary graph of the average L5 ET EPSC amplitudes in response to L1–4 thalamocortical maximal photostimulation in 1d SE vs. 1d NE, or 7d SE vs. 7d NE mice (1d SE: 8 cells/3 mice; 1d NE: 7 cells/4 mice; 7d SE: 9 cells/5 mice; 7d NE: 12 cells/4 mice).

(G) Representative traces of L5 ET EPSCs in response to L1–4 thalamocortical maximal photostimulation in 1d SE (G₁, black) vs. 1d NE (G₁, red), 7d SE (G₂, black) vs 7d NE (G₂, red) mice.

Detailed statistical values are listed in Table 1.