Figure 7. Transient shift in ET sound response properties one day after NE.

(A) Example two-photon field of view from ETs imaged before and after exposure.

(B) Average responses to pure tones across all ETs in SE mice (−1d: n=385 neurons; 1d: n=414 neurons; 7d: n=400 neurons).

(C) Same as in (A) for NE mice (−1d: n=268 neurons; 1d: n=235 neurons; 7d: n=296 neurons). Asterisks indicate significant pairwise differences (Bonferroni corrected for multiple comparisons).

(D) Tuning curves aligned to best frequency (BF) in SE mice.

(E) Same as in (D) for NE mice. Asterisks indicate significant pairwise differences (*p<0.05, ****p<0.0001, Bonferroni corrected for multiple comparisons).

(F) Change in BF of matched cells in SE (black) and NE (red) mice (1d SE: n=68 neurons; 1d NE: n=54 neurons; 7d SE: n=68 neurons; 7d NE: n=71 neurons). Asterisks indicate significant pairwise differences (*p<0.05, ****p<0.0001, Bonferroni corrected for multiple comparisons).

(G) Accuracy of a multinomial logistic regression classifier trained to decode the frequency of pure tones at 80 dB SPL. Dashed line represents chance level. Error bars represent standard deviation of decoding iterations. Asterisks indicate significant pairwise differences (*p<0.05, ****p<0.0001, bootstrap test Bonferroni corrected for multiple comparisons).

(H) Average responses to white noise bursts at different intensities across all ETs in SE mice (−1d: n=331 neurons; 1d: n=270 neurons; 7d: n=299 neurons).

(I) Same as (H) in NE mice (−1d: n=226 neurons; 1d: n=222 neurons; 7d: n=252 neurons). Asterisks indicate significant pairwise differences (Bonferroni corrected for multiple comparisons).

(J) Change in intensity thresholds across days in SE (black) and NE (red) mice (1d SE: n=42 neurons; 1d NE: n=49 neurons; 7d SE: n=50 neurons; 7d NE: n=51 neurons). Asterisks indicate significant pairwise differences (*p<0.05, **p<0.01, Bonferroni corrected for multiple comparisons).

(K) Accuracy of a multinomial logistic regression trained to decode sound intensity. Dashed line represents chance level. Error bars represent standard deviation of decoding iterations. Asterisks indicate significant pairwise differences (*p<0.05, bootstrap test Bonferroni corrected for multiple comparisons).

Detailed statistical values are listed in Table 1.