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. 2005 Aug;43(8):3699–3703. doi: 10.1128/JCM.43.8.3699-3703.2005

FIG. 2.

FIG. 2.

Representative results for patterns obtained with the Genotype MTBDR assay. The positions of the oligonucleotides and the marker line are given on the left side. The specificity and targeted genes of the lines are shown from top to bottom as follows: conjugate control; amplification control (23S rRNA); M. tuberculosis complex-specific control (23S rRNA); control of rpoB amplification 5 to 9; rpoB wild-type probes located in the 81-bp hot spot region (the localization is shown in Fig. 1); rpoB mutant (Mut) probes with mutations in codons 516, 526, and 531; control of katG amplification; katG codon 315 wild-type probe; and katG codon 315 mutation probes (sequences in parenthesis). Along the bottom is a band for orientation of the strip. Patterns of the strips for MDR and susceptible M. tuberculosis strains are shown on the right. Lane 1, negative control; lane 2, H37Rv (wild type); lane 3, rpoB D516V, katG S315T1; lane 4, rpoB H526Y, katG S315T1; lane 5, rpoB H526D, katG S315T1; lane 6, rpoB S531L, katG S315T1; lane 7, rpoB H526N, katG S315T1; lane 8, rpoB 514-to-516 deletion, katG S315T1.