TABLE 2.
Nucleotide sequences of PCR primers used to amplify the C. dubliniensis FUR1 and FCY1 genesa
| Primer | Sequence (5′ to 3′) | Nucleotide coordinatesb |
|---|---|---|
| FCY1F | CGGCATATTAATTCCGCTTG | −145 to −164 |
| FCY1R | CGAATTCTCTTCTGCTTCTG | +106 to +125 |
| FUR1F | ATGTGGGTTACATCAGAAGA | −198 to −217 |
| FUR1R | TCTCGCGACCCTCCTCTAAC | +205 to +224 |
a
The nucleotide sequences of the C. dubliniensis FUR1 and FCY1 genes were obtained from the C. dubliniensis genome sequence database (http://www.sanger.ac.uk/Projects/C_dubliniensis/).
b
Nucleotide coordinates for C. dubliniensis genes FUR1 and FCY1 have the first bases of the ATG start codons designated +1.