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. 2005 Aug;43(8):3755–3759. doi: 10.1128/JCM.43.8.3755-3759.2005

FIG. 3.

FIG. 3.

(a) Detection of B. equi-positive salivary glands from individual B. microplus adults fed as nymphs on a chronically infected horse. (b) Confirmation of amplicon identity of positive B. equi salivary glands from a duplex nested PCR with a digoxigenin-labeled ema-1 probe. SG1 to SG11, DNA amplified from salivary glands of individual ticks; H072, DNA amplified from B. equi Florida isolate-infected erythrocytes as a positive control. The predicted amplicon sizes for B. equi ema-1 and B. microplus α-tubulin are indicated in the right margin. Molecular size markers are in the left margin.