TABLE 1.
Positions and nucleotide sequences of oligonucleotide primers
| Primera | Polarity | Nucleotide position | Nucleotide sequence (5′-3′) |
|---|---|---|---|
| RD | |||
| RD037 | Sense | 2008-2027 | GCAGCAGCATATGGATATGT |
| RD038 | Antisense | 2258-2277 | TGACTGTGCTAAAGCCTCTA |
| RD051 | Sense | 2061-2080 | CATACACATGAATGCCAGGA |
| RD052 | Antisense | 2238-2257 | GTACTTCTTGCTGGTGAAAT |
| TT | |||
| TT6 | Sense | 1900-1919 | ACAGACAGAGGAGAAGGCAA |
| TT7 | Antisense | 2211-2228 | TACCATTTAGCTCTCATT |
| TT8 | Sense | 1918-1938 | AACATGTTATGGATAGACTGG |
| TT9 | Antisense | 2166-2185 | CTGGCATTTTACCATTTCCA |
| NG | |||
| NG054 | Sense | 3-22 | TTTGCTACGTCACTAACCAC |
| NG147 | Antisense | 211-233 | GCGAGTCCCCGAGCCCGAATTGCC |
| NG133 | Sense | 91-115 | GTAAGTGCACTTCCGAATGGCTGA |
| NG132 | Antisense | 204-223 | AGCCCGAATTGCCCCTTGAC |
| TLMV-S | |||
| TLMV-S1 | Sense | 30-48 | ATTTGAATTGCCGACCACA |
| TLMV-S2 | Antisense | 298-319 | CGCCAGACTGATCTAGCCCGAA |
| TLMV-S3 | Sense | 36-55 | ATTGCCGACACAAACTGAC |
| TLMV-S4 | Antisense | 284-305 | AGCCCGAATTGCCCCTAGTC |
| TLMV-L | |||
| LS2 | Sense | 33-51 | WGAATTGCCGACCACAAAC |
| LA2 | Antisense | 311-330 | AAGTTTCTTGCCCGKTCCGC |
| LA3 | Antisense | 280-299 | AGCCCGAATTGCCCCTAGAC |
a
A genotype 21-specific primer set (16) was not used in this study.