FIG. 2.

Triplex dipstick detection of individual and multiple virus amplicons. Nucleic acid standards for HBV, HCV, and HIV-1 were individually amplified by RT-PCR and then tested with the dipstick to determine its ability to identify individual viruses. In addition, a mixture of plasma specimens from three individuals, each infected with one of the three viruses, was used to test the ability of the triplex dipstick to detect HBV DNA, HCV RNA, and HIV-1 RNA in the same sample simultaneously. The viral loads of the combined sample are indicated. A negative plasma sample (Neg) was used to test for the specificity of triplex dipstick detection.