FIG. 1.

Antigens used for analysis of seroconversion in children with CF. The indicated purified proteins (1 μg), ExoS (S), PcrV (V), ETA (A), PopB (B), and a P. aeruginosa PA01 cell lysate, were subjected to SDS-polyacrylamide gel electrophoresis. One gel (upper panel) was stained with Coomassie blue. Molecular weight (MW) markers are shown in the left lane. Proteins from two other gels were transferred to PVDF membranes and incubated with sera from two patients with CF. The membrane was then probed with goat α-human IgG-HRP and developed by enhanced chemiluminescence. Exposed X-ray films are shown (middle and lower panels).