TABLE 1.

PCR primers used in this study

Primer designation	Sequence	Primer locationa	Reference or source
toxB.911F	5′-ATA CCT ACC TGC TCT GGA TTG A-3′	79431-79452	30
toxB.1468R	5′-TTC TTA CCT GAT CTG ATG CAG C-3′	80030-80009	30
toxB 5′int-up	5′-TTT TCG CGT AAT GAT GC-3′	81278-81294	This study
toxB 5′int-lo	5′-ACG CCG TGA GAA TAA TGT C-3′	81954-81936	This study
toxB 3′int-up	5′-CAA CAG CCC CTT CAT TCC ATT C-3′	83708-83729	This study
toxB 3′int-lo	5′-TTT TGC CAC ATT GCT AAG ATA ACG-3′	84277-84254	This study
toxB 5′ upper	5′-AAA ATA ATT CAT CCC CCA GTT CT-3′	78906-78298	27
toxB 5′ lower	5′-CCG CAC CAA AGG CAT TAG-3′	79472-79455	27
toxB 3′ upper	5′-TAG CGG AAA GAA TAT TGG TAG TCA-3′	84703-84726	27
toxB 3′ lower	5′-CTG TAG TGT GGC GGG AAC G-3′	85555-85537	27

^aPrimer locations refer to nucleotides in the pO157 toxB sequence (GenBank accession no. AF074613).