FIG. 4.

RT-PCR analysis of C. perfringens strains. (A) Total RNA prepared from each specified strain was subjected to RT-PCR analysis using cpb2-specific internal primers as described in Materials and Methods. RT (+) and RT (−) indicate the presence and absence, respectively, of RT in the PCR. The RT-PCR-amplified products were analyzed by agarose (1%) gel electrophoresis and photographed under UV light. Molecular sizes of the DNA markers are given on the right. (B) Total DNA isolated from each specified strain was subjected to PCR analysis using the same cpb2-specific internal primers as those used for RT-PCR. The PCR-amplified products were analyzed by agarose (1%) gel electrophoresis and photographed under UV light. Molecular sizes of the DNA markers are given on the left.