FIG. 1.

Detection of the C. guilliermondii clade by using two probes, illustrating that only clade species are positive for both probes. Measurement in fluorescence units was adjusted by subtracting the mean of values for water blank samples as background. All C. guilliermondii var. carpophila and C. fermentati strains gave signals for both probes within the range established by the two C. guilliermondii strains shown. Pichia triangularis gave the highest recorded signal of both probes for a nontarget species. The two signals were 37% and 18% of the lowest positive signal and therefore were considered negative for both probes.