TABLE 4.
Primer | Species or strain identified | Primer sequence | Probe signal range (AFU)
|
Min ratio of signalsc | |
---|---|---|---|---|---|
Negativea | Positiveb | ||||
AlbA | Candida albicans | GATGAGATGACCCGGGTCTG | 0-118 | 926-2,991 | 7.9 |
AnomA | Pichia anomala | TTGAATGTGGCTTCACTTCG | 0-236 | 2,117-2,609 | 9.0 |
DelphinA | Kluyveromyces delphensis | ACTCCGGGAATGTAGCTGTC | 0-317 | 5,262 | 16.6 |
DubA1 | C. dubliniensis | GATGAGATGGCCCGGGTCTA | 0-130 | 2,792-3,556 | 21.5 |
FabiA1 | P. fabianii | CAAGATAATAGCTTGGGAAT | 0-715d | 2,979 | 4.2 |
GlabC | C. glabrata | AGAGTACCACTTTGGGACTG | 0-46 | 1,026-1,545 | 22.3 |
HaemA | C. haemulonii | GAGCAGTCGATGTAGTACAG | 0-52 | 239-909 | 4.6 |
HaemIIA | C. haemulonii type II | GGAACTAAAAGTGGGAGCTG | 0-71 | 347-1,402 | 4.9 |
HellenA | Zygoascus hellenicus | GTTTATGGTGAAGAACCCAC | 0-112 | 2,075 | 18.5 |
JadB2 | P. jadinii | GGCAGTGTGTTTATAGCCCC | 0-660e | 1,356 | 2.1 |
LelonA | Lodderomyces elongisporus | AGCGGTAGGAGAATTGCGTA | 0-227 | 2,804-6,270 | 12.4 |
LipoA | Yarrowia lipolytica | GAAATGAGTGGAGAGTGGCC | 0-185 | 2,301 | 12.4 |
LusitA | Clavispora lusitaniae | GGAAAGAATGTGGCGCGTGC | 0-130 | 2,949 | 22.7 |
MaltA | C. maltosa | CCACTGTCGATACTGCCAGT | 0-265 | 4,911 | 18.5 |
NeerA | C. neerlandica | TAGGAGAATCGCTTGGGAAC | 0-36 | 2,010 | 55.8 |
OccidA | Issatchenkia occidentalis | GTTTTTGCCGCAGGAGAAGGC | 0-117 | 1,481-2,316 | 12.7 |
OnychA | P. onychis | TCTGGTTGTGCCTGGCGCAG | 0-62 | 2,478-2,762 | 40.0 |
ParapB50 | C. parapsilosis | AGTTTGAGCGGTAGGATAAG | 0-201 | 2,673-3,893 | 13.3 |
PciferB2 | P. ciferrii | TATAGTTTCTGTTGATATTG | 0-364 | 1,298 | 3.6 |
PeterA | P. petersonii | CCGTGTGGCGGGGAGCCCGGT | 0-43 | 1,455 | 33.8 |
PseudoA | C. pseudointermedia | AGCCCCGTAGGTACCACAAC | 0-48 | 1,621 | 33.8 |
SciferB | Stephanoascus ciferrii | CAAAAGAATGTGGCTCCGCT | 0-223 | 658 | 3.0 |
SfariA | S. farinosus | CAACTTTGGAGACGGCCTTG | 0-68 | 2,624 | 38.6 |
SojA | C. sojae | GGCGGTAGGAGAATTGCGA | 0-82 | 1,378-1,585 | 16.8 |
SsmithA | S. smithiae | GCCTAGGTTGATACTACCTA | 0-58 | 1,068-1,628 | 18.4 |
WojA | Candida sp. strain NRRL Y-17456 | TGGGCGGTAGGACAATTGCA | 0-198 | 2,829-3,063 | 14.3 |
VisA2 | C. viswanathiif | GGCGGCAGGACAATCGCGT | 0-109 | 1544-2096 | 14.2 |
UniB | All species | TCGAGTTGTTTGGGAATGCA | NAg | 1050-3760 | NA |
Range of adjusted fluorescence units for all strains negative for the given probe.
Range of AFU for all strains positive for the given probe.
The min ratio is the lowest recorded positive value divided by the highest recorded negative value.
P. veronae and C. blankii cross-reacted with FabiA1. All other strains tested gave less than 112 AFU.
C. lodderae NRRL Y-17317, Pichia sp. strain NRRL Y-11569, and C. viswanathii NRRL Y-27370 cross-reacted with JadB2. All other strains gave less than 109 AFU.
C. viswanathii includes C. lodderae.
NA, not applicable.