TABLE 4.

ASPE species-specific primers

Primer	Species or strain identified	Primer sequence	Probe signal range (AFU)		Min ratio of signalsc
			Negativea	Positiveb
AlbA	Candida albicans	GATGAGATGACCCGGGTCTG	0-118	926-2,991	7.9
AnomA	Pichia anomala	TTGAATGTGGCTTCACTTCG	0-236	2,117-2,609	9.0
DelphinA	Kluyveromyces delphensis	ACTCCGGGAATGTAGCTGTC	0-317	5,262	16.6
DubA1	C. dubliniensis	GATGAGATGGCCCGGGTCTA	0-130	2,792-3,556	21.5
FabiA1	P. fabianii	CAAGATAATAGCTTGGGAAT	0-715d	2,979	4.2
GlabC	C. glabrata	AGAGTACCACTTTGGGACTG	0-46	1,026-1,545	22.3
HaemA	C. haemulonii	GAGCAGTCGATGTAGTACAG	0-52	239-909	4.6
HaemIIA	C. haemulonii type II	GGAACTAAAAGTGGGAGCTG	0-71	347-1,402	4.9
HellenA	Zygoascus hellenicus	GTTTATGGTGAAGAACCCAC	0-112	2,075	18.5
JadB2	P. jadinii	GGCAGTGTGTTTATAGCCCC	0-660e	1,356	2.1
LelonA	Lodderomyces elongisporus	AGCGGTAGGAGAATTGCGTA	0-227	2,804-6,270	12.4
LipoA	Yarrowia lipolytica	GAAATGAGTGGAGAGTGGCC	0-185	2,301	12.4
LusitA	Clavispora lusitaniae	GGAAAGAATGTGGCGCGTGC	0-130	2,949	22.7
MaltA	C. maltosa	CCACTGTCGATACTGCCAGT	0-265	4,911	18.5
NeerA	C. neerlandica	TAGGAGAATCGCTTGGGAAC	0-36	2,010	55.8
OccidA	Issatchenkia occidentalis	GTTTTTGCCGCAGGAGAAGGC	0-117	1,481-2,316	12.7
OnychA	P. onychis	TCTGGTTGTGCCTGGCGCAG	0-62	2,478-2,762	40.0
ParapB50	C. parapsilosis	AGTTTGAGCGGTAGGATAAG	0-201	2,673-3,893	13.3
PciferB2	P. ciferrii	TATAGTTTCTGTTGATATTG	0-364	1,298	3.6
PeterA	P. petersonii	CCGTGTGGCGGGGAGCCCGGT	0-43	1,455	33.8
PseudoA	C. pseudointermedia	AGCCCCGTAGGTACCACAAC	0-48	1,621	33.8
SciferB	Stephanoascus ciferrii	CAAAAGAATGTGGCTCCGCT	0-223	658	3.0
SfariA	S. farinosus	CAACTTTGGAGACGGCCTTG	0-68	2,624	38.6
SojA	C. sojae	GGCGGTAGGAGAATTGCGA	0-82	1,378-1,585	16.8
SsmithA	S. smithiae	GCCTAGGTTGATACTACCTA	0-58	1,068-1,628	18.4
WojA	Candida sp. strain NRRL Y-17456	TGGGCGGTAGGACAATTGCA	0-198	2,829-3,063	14.3
VisA2	C. viswanathiif	GGCGGCAGGACAATCGCGT	0-109	1544-2096	14.2
UniB	All species	TCGAGTTGTTTGGGAATGCA	NAg	1050-3760	NA

^aRange of adjusted fluorescence units for all strains negative for the given probe.

^bRange of AFU for all strains positive for the given probe.

^cThe min ratio is the lowest recorded positive value divided by the highest recorded negative value.

^dP. veronae and C. blankii cross-reacted with FabiA1. All other strains tested gave less than 112 AFU.

^eC. lodderae NRRL Y-17317, Pichia sp. strain NRRL Y-11569, and C. viswanathii NRRL Y-27370 cross-reacted with JadB2. All other strains gave less than 109 AFU.

^fC. viswanathii includes C. lodderae.

^gNA, not applicable.