TABLE 5.
ASPE multispecies primers
| Primer | Species identified | Primer sequence | Cross-reactivitya | Positive rangeb | No. of species cross-reacting |
|---|---|---|---|---|---|
| AtellA | Kazachstania telluris, K. bovina, K. heterogenica, K. pintolopesii | TGTAATTTGGAGAGTGGATC | 0-151 | 523-1,080 | 0 |
| GuilA | C. fermentati, C. guilliermondii var. guilliermondii, C. guilliermondii var. carpophila | CAGACTCGATATTTTGTGAG | 0-226 | 1,569-5,145 | 0 |
| HansenA | Debaryomyces hansenii var. hansenii, D. hansenii var. fabryi, D. nepalensis, C. psychrophila | GACTAAGGAATGTGGCTCTA | 0-719 | 1,414-4,414 | 1c |
| KrusD | C. krusei, C. pseudointermedia | CTTGGAACAGGGCGCCCAG | 0-79 | 2,923-4,724 | 0 |
| Trop/ElongA | C. tropicalis, Lodderomyces elongisporus | GGCGGTAGGAGAATTGCGT | 0-1,049 | 2,150-5,189 | 1d |
| ZeylaG | C. zeylanoides, C. santamariae var. santamariae, C. santamariae var. membranifaciens | GACATTGGAATGTAGCTTTA | 0-121 | 461-1,412 | 0 |
a
Range of fluorescence values for all strains that cross-react with the given probe. Values less than 40% of the lowest positive value are not considered to be cross-reactive.
b
Range of fluorescence values for all strains positive for the given probe.
c
Cross-reacts with C. sake.
d
Cross-reacts with eight strains of C. guilliermondii and one strain of C. fermentati.