TABLE 2.

Primers used for pXO2-A locus test of the VX2-3 locus shown in the plasmid map^a

PCR no.	Primer	Sequence (5′ to 3′)	5′ positionb	Predicted length (bp) for B. anthracis
24	406-f	+CAC GAA AGT TTA AAT TGT ACC GCA GA	60590	506
	407-r	−AGA GTT ATT GGA AAA CCA GGA GAT AC	61095
27	462-fam-f	+TCA ACT TAA GTA AAT TCT CCT TTT CCA	60663	232 (fam)
	464-r	−CCC ATC GAA CGT GTT CTA GCT ACA GTA	60894
28	462-fam-f	TCA ACT TAA GTA AAT TCT CCT TTT CCA	60663	330 (fam)
	420-r	−CTG CTA TGG ATC AAA AAT GGA TAG TA	60992
29	463-hex-f	+TCA ACT TAA GTA AAT TCT CCT TTT CCA	60663	330 (hex)
	420-r	−CCC ATC GAA CGT GTT CTA GCT ACA GTA	60894

^aPlasmid map shown in Fig. 1.

^bNucleotide positions refer to B. anthracis (accession number AF188935).