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Southern hybridization with a digoxigenin-labeled probe to the ORF sequence containing spn9802 (A) and spn9828 (B) of S. pneumoniae WU2. Chromosomal DNAs from S. pneumoniae WU2 (lane 1), S. mitis 903 (lane 2), atypical clinical streptococci (lane 3 to 22), and clinical isolated pneumococci (lane 23 to 26) were digested by either PstI (A) or NcoI (B). The lack of clear results in lanes 3 to 20 is due to negative reactions to the digoxigenin-labeled probes.
