TABLE 1.
Sequences of oligonucleotide primers
| Primer | Sequence | Amplicon size (bp) |
|---|---|---|
| lytA sense | 5′-CAA CCG TAC AGA ATG AAG CGG-3′ | 308 |
| lytA antisense | 5′-TTA TTC GTG CAA TAC TCG TGC G-3′ | |
| ply sense | 5′-ATT TCT GTA ACA GCT ACC AAC GA-3′ | 329 |
| ply antisense | 5′-GAA TTC CCT GTC TTT TCA AAG TC-3′ | |
| Spn9802-143F | 5′-CAA GTC GTT CCA AGG TAA CAA GTC T-3′ | 162 |
| Spn9802-304R | 5′-CTA AAC CAA CTC GAC CAC CTC TTT-3′ | |
| Spn14.1-27F | 5′-CAC CAT CAT AAA TGC TAG CCT TGA-3′ | 301 |
| Spn14.1-327R | 5′-TTG GAA GAT AGA ACC ATA GTT GCT TG-3′ | |
| Spn9822-17F | 5′-CAG GGA TTC GCT TAA TAG CTG GT-3′ | 202 |
| Spn9822-218R | 5′-CCT GCC AAG GTC AAT CCA TAA A-3′ | |
| Spn24-4F | 5′-TGT CCC AAT CTC TAA AGG ATG GAG-3′ | 225 |
| Spn24-228R | 5′-CAA CTT GCC CGA TTC ACT TTT C-3′ | |
| Spn25.1-12F | 5′-TAA CCA AAA CCT TTA GTT TAG GTT GAT TTT-3′ | 135 |
| Spn25.1-146R | 5′-CTC ACT ATT TAG AGA CTC TTG AGG TTT GTT-3′ | |
| Spn25.2-6F | 5′-GAG ACT CCG TGG AAC CTC TTT G-3′ | 130 |
| Spn25.2-135R | 5′-GAC GAA CTG CCA TAT AAG AAC CAT T-3′ | |
| Spn27-11F | 5′-CAA GAT TGA ACG TAT CCG CGA-3′ | 285 |
| Spn27-295R | 5′-TGG CCA GTT GAT TCG AGT TTG-3′ | |
| Spn9827-70F | 5′-TGG CCC TAA TAC AAC GAT GGA-3′ | 208 |
| Spn9827-277R | 5′-AGC AAG TGA GGG AAA GAC CGT-3′ | |
| Spn9828-19F | 5′-GGC ATT GTG AAT GGA TTG ATT G-3′ | 227 |
| Spn9828-245R | 5′-TCA TGT GCA TCC CAA GCT ACA-3′ | |
| Spn34-5F | 5′-GGG CCT GTA TCG TTC TAC TAG GG-3′ | 231 |
| Spn34-235R | 5′-TTT CTC TCC AAT CTA CCT TAC CCG-3′ | |
| Spn38-5F | 5′-AAT GGT CGT ATT GTT GCC GG-3′ | 266 |
| Spn38-270R | 5′-AAG TAA TCA ATC GCA TCT TGT AGG G-3′ | |
| Spn53-415F | 5′-GGG ATT CAA AAT ACA GTA GAG TCC TTG-3′ | 346 |
| Spn53-760R | 5′-TGA GCT TTG CAG CAT AAC GG-3′ | |
| Spn63-191F | 5′-GGA CTT TCT TTT TCC CAT CGG-3′ | 339 |
| Spn63-529R | 5′-CCC AGA TTT GTT TGA GCC AAC-3′ | |
| Spn66-10F | 5′-AAC AAT CGA ATC CCC TGG G-3′ | 312 |
| Spn66-321R | 5′-AAC TCC ACC AGT CAG GGA TGA-3′ | |
| Spn70-49F | 5′-CCT GCG TAG CCC ATA TCT CG-3′ | 253 |
| Spn70-301R | 5′-GGC AGG ATG CCA TTC GAC T-3′ | |
| Spn76-33F | 5′-CAT AGA TTC CCC AGA TCT CGG T-3′ | 351 |
| Spn76-383R | 5′-CAG GTC AGA GTG AGC TTT TGG TG-3′ | |
| Spn79-68F | 5′-CGC TAG TGA TTG GTG GTG AGC-3′ | 261 |
| Spn79-328R | 5′-CAA GTT CCT GCA AGG TTT CAA GA-3′ | |
| Spn84-93F | 5′-CTT GTC AGC CTT TGC TTG GAA-3′ | 332 |
| Spn84-424R | 5′-GCG CTA GAC AGA TCA GCA GGA-3′ | |
| Spn86-24F | 5′-CGA AAC ACC AGC AGA AGA GCT-3′ | 184 |
| Spn86-207R | 5′-AAT TTC GCC ACT ATT TAG CCA GAT-3′ |