TABLE 3.
Specificity of the primers designed from subtractive DNA
| Primer set | Result for type strain
|
Result for atypical oral streptococcal strains
|
Result for clincal pneumococcal isolate:
|
|||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WU2 | 903 | 1a | 2 | 3 | 4 | 5 | 6a | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14a | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | |
| lytA | + | − | − | + | − | − | − | − | − | − | − | − | − | − | − | − | + | − | − | − | − | + | + | + | + | + |
| ply | + | − | + | − | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | + | − | + | + | + | + |
| Spn9802b | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | + | + | + |
| Spn9822 | + | − | − | − | − | − | − | − | − | − | − | − | − | − | + | − | + | − | + | + | − | + | + | + | + | + |
| Spn9827 | + | − | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | + | + |
| Spn9828b | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | + | + | + |
| Spn14.1 | + | − | − | + | − | − | − | − | − | − | − | − | − | − | + | + | − | + | − | − | + | − | + | + | + | + |
| Spn24 | + | − | + | − | + | + | + | − | + | + | + | + | + | + | − | + | + | + | − | − | − | + | + | + | + | + |
| Spn25.1 | + | − | − | − | + | + | + | − | + | + | − | + | − | − | − | − | − | − | − | − | − | + | + | + | + | + |
| Spn25.2 | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | + | − | − | − | − | − | − | − | − | − |
| Spn27 | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | − | + | − | − | − | − | + | + | + | + |
| Spn34 | + | − | + | − | − | − | − | + | − | − | − | − | + | − | − | − | − | − | − | − | − | − | + | + | + | + |
| Spn38 | + | − | + | + | + | + | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| Spn53 | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | − | − | − | − | + | + | + | + |
| Spn63 | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| Spn66 | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| Spn70 | + | − | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| Spn76 | + | − | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | + | + | + | + | + | + | + | + |
| Spn79 | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | + |
| Spn84 | + | − | + | + | + | + | + | + | + | + | + | + | + | − | + | + | − | + | + | + | + | − | + | + | + | + |
| Spn86 | + | − | + | − | + | + | + | + | + | + | − | + | + | − | + | + | + | + | + | + | + | + | + | + | + | + |
a
Strains 1, 6, and 14 are classified as S. oralis on the basis of phenotypic tests and API biotyping and additional biochemical tests. The other strains are classified as S. mitis.
b
No amplifying DNA from any atypical streptococci tested but amplifying DNA from all typical and clinically isolated pneumococci tested.