TABLE 1.

List of oligonucleotide primers used in the multiplex PCR

Primer	Sanger's strain no. or GenBank accession no.b	Primer sequence (5′-3′)	Nucleotide positionc	Product size (bp)	Primer product concn (μM)
3-f	Z47210	ATG GTG TGA TTT CTC CTA GAT TGG AAA GTA G	9179	371	1.5
3-r		CTT CTC CAA TTG CTT ACC AAG TGC AAT AAC G	9519
6A/B-f (biotin)	AF316640	AAT TTG TAT TTT ATT CAT GCC TAT ATC TGG	8656	250	0.5
6A/B-r		TTA GCG GAG ATA ATT TAA AAT GAT GAC TA	8877
19A-fa	AF094575	GTT AGT CCT GTT TTA GAT TTA TTT GGT GAT GT	12118	478	1.0
19A-r		GAG CAG TCA ATA AGA TGA GAC GAT AGT TAG	12566
22F-f	1772/40 (da)	GAG TAT AGC CAG ATT ATG GCA GTT TTA TTG TC	11055	643	1.5
22F-r		CTC CAG CAC TTG CGC TGG AAA CAA CAG ACA AC	11666

^aPrimer previously published as in reference 1.

^bPrimers were designed using the sequence from either GenBank (accession numbers provided) or www.sanger.ac.uk (strain numbers provided).

^cStart position of each primer.