Spearman rank correlation analysis between OD values from the EIA and the intensity of agglutination from the LBAA utilizing ESAT-6 (A) or rMPB70 (B) and between OD values and the intensity of the test line band from the ICGA with rMPB70 (C). The EIA and LBAA turned out to be positively correlated (P < 0.0001), with r = 0.73 (95% CI, 0.70 to 0.77) or 0.68 (95% CI, 0.64 to 0.72) when using ESAT-6 or rMPB70 as the detecting antigen, respectively. A statistically significant correlation between the ICGA and EIA utilizing rMPB70 was also found, with r = 0.87 (95% CI, 0.85 to 0.88, P < 0.0001).