TABLE 1.

A summary of the Entamoeba histolytica isolates analyzed^a

Isolate	Country of origin	Date of isolation (mo/yr)	Source	Clinical diagnosis	Stool microscopy	DNA origin
HM-1:1MSSb (r)	Mexico	1967	M	Colitis	NA	Axenicd
200:NIHb	India	1949	M	Colitis	NA	Axenicd
HK9b	Korea	NA	M	Colitis	NA	Axenicd
Rahmanb	England	1972	M	Asymptomatic	NA	Axenicd
DS2-3497c	Bangladesh	09/2000	F	Colitis	Negative	Xenice
DS1-1036c	Bangladesh	10/2000	M	Colitis	Positive	Xenice
DS17-27c	Bangladesh	06/2001	M	Colitis	Positive	Xenice
DS6-64c	Bangladesh	04/2001	M	Colitis	Negative	Xenice
DS3-15c	Bangladesh	05/2001	F	Colitis	Negative	Xenice
MS6-21c	Bangladesh	11/1999	M	Asymptomatic	NA	Xenice
MS53-3046c	Bangladesh	10/2003	F	Asymptomatic	NA	Xenice
MS27-291c	Bangladesh	06/2001	F	Asymptomatic	NA	Xenice
MS30-1047c	Bangladesh	07/2001	M	Asymptomatic	NA	Xenice
MS15-3394c	Bangladesh	08/2000	F	Asymptomatic	NA	Xenice

^a(r), reference strain; NA, not available; M, male; F, female. ATCC accession numbers: HM-1:IMSS, 30459; 200:NIH, 30458; HK9, 30015; Rahman, 30886.

^bConfirmed by PCR analysis of the rRNA episome, strain-specific gene, and PCR and restriction fragment length polymorphism of the serine-rich E. histolytica protein (4).

^cPositive for an E. histolytica-specific fragment of the rRNA gene (4).

^dGenomic DNA isolated following standard protocols (9,16).

^eGenomic DNA amplified using GenomiPhi DNA amplification (Amersham Biosciences) prior to PCR analysis.