TABLE 2.

The genes, primer sequences, and size of the PCR product utilized for the study are listed^a

Gene, genetic material, or protein	Primer sequences	PCR product (bp)	Reference
E. histolytica-specific rRNA	5′-GGCCAATTCATTCAATGAATTGAG-3′	850	4
	5′-CTCAGATCTAGAAACAATGCTTCT-3′
ssu rRNA	S: TGA GTTAGGATGCCACGACA	1560	GenBank accession number X56991
	AS: GTA CAAAGGGCAGGGACGTA
Actin	S: GGGAGACGAAGAAGTTCAAGC	1042	GenBank accession numbers M16339, M16396, M16340 and M16341 and TIGR genome database loci 35.m00215, 74.m00188, and 597.m00020
	AS: GGCAAGAATTGATCCTCCAA
γ-Tubulin	S: TGTTGGACAATGTGGAAATCA	1000	GenBank accession number U20322
	AS: AGCCAATAACATCCCACTCA
cpn60	S: ATGGGACAACAACAGCAACA	1124	GenBank accession number AF029366
	AS: TCCTCCATCAATTCCTGCAT
Lectin (hg13)	S: GACATATGCAACAAAAACTGAAGC	900	GenBank accession number L14815 and TIGR genome database loci 29.m00206 and 290.m00068
	AS: ACACTTGGTTTTCACTTTACGT
Amebapore C	S: AAGGTAAATTGAATCAAAACACAAA	928	TIGR scaffold number 00018
	AS: TCGACCGTTTGTTTACTTCTCA
rabE	S: GGGGAAGGAAAAGTTGGAAA	632	GenBank accession number AB054581
	AS: TCAGATTTTGCTTGACGAGTG
Cysteine proteinase 5	S: TTTCAATACTTGGGTTGCAAAT	885	GenBank accession number X91644
	AS: GCAGCTCCTGAAGCAATACC
Intron Cta	S: CTGCATTATCTCAATTTGTTCC	500 (Intron     size, 59)	19a
	AS: GGATCTTCATTAATTCTAATTG
Intron cdc2c	S: GCATGGGACACAGTCTG	279 (Intron     size, 61)	19a
	AS: GGCAAAAAGCAAGTCCTTG
Intron 64.m00165	S: ATTTATTGGCTTGTGCGTGA	700 (Intron     size, 400)	TIGR locus number 64.m00165
	AS: TGGTGATGAACATCCTCAAAA
Intron 128.m00017b	F: GCTTTTGTCTCTTCACAAATACTTCA	835 (Intron     size, 700)	TIGR locus number 128.m00017
	R: TTGTTCTGGTGTTAATCCATCG
Intergenic region between 2.m00567 and 2.m00568	S: AAAAGCCATTGAAAATGGATG	668	TIGR locus numbers 2.m00567 and 2.m00568
	AS: TCCATCAACATCCAATACAATTG
Locus 1-2	S: CTGGTTAGTATCTTCGCCTGT	396-500	7
	AS: CTTACACCCCCATTAACAAT
Locus 5-6	S: CTAAAGCCCCCTTCTTCTATAATT	396-550	7
	AS: CTCAGTCGGTAGAGCATGGT
Chitinase	S: GGAACACCAGGTAAATGTATA	250-600	7
	AS: TCTGTATTGTGCCCAATT

^aS, sense; AS, antisense; F, forward; R, reverse.

^bIntron confirmed by Northern blot analysis (Ryan C. MacFarlane, personal communication).