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Reaction patterns of adenovirus antigens with human sera (1:102 diluted) on NC membrane. (A) Line assay of sprayed antigens. (B) Immunoblot assay of recombinant adenovirus fiber and pIX antigens separated by electrophoresis, as well as sprayed HAdV-5 hexon and reaction control. The minimal differences in the position of each protein band from one NC strip to another based on different polyacrylamide gels are bordered. The adenovirus antigens and controls are indicated on the right side.
