Species-level identification and drug resistance determination. Real-time PCR fluorescent signatures are associated with several bacterial species and their resistance determinants. Each sample was probed with four different molecular beacon probes in one reaction and analyzed using the Stratagene Mx4000 software. Symbols: squares, mecA; triangles, spa; diamonds, SG16S; circles, vanA. dR, first derivative of the fluorescence changes with respect to temperature.