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Detection of S. aureus virulence factor PVL in MRSA isolates. Real-time PCR signatures for a PVL-positive MRSA and a PVL-negative MRSA when probed for lukF are shown. PVL-positive MRSA (squares) and PVL-negative MRSA (circles) were probed for lukF in two separate reactions and compared using the Stratagene Mx4000 software. dR, first derivative of the fluorescence changes with respect to temperature.
