TABLE 1.
Molecular beacon and primer sequences
| Oligonucleotidea | Sequence (5′-3′)b |
|---|---|
| mecA, F | TGGTATGTGGAAGTTAGATTGG |
| mecA, R | ATATGCTGTTCCTGTATTGGC |
| mecA, MB | (FAM)-CGCGATTTCAATATGTATGCTTTGGTCTTTCTGATCGCG-(DABCYL) |
| spa, F | CATTACTTATATCTGGTGGCG |
| spa, R | GTTAGGCATATTTAAGACTTG |
| spa, MB | (CY5)-CGCGAGTTAGGCATATTTAAGACTTGTCGCG-(BHQ-2) |
| SG16S, F | TGGAGCATGTGGTTTAATTCGA |
| SG16S, R | TGCGGGACTTAACCCAACA |
| SG16S, MB | (HEX)-CGCTGACTTACCAAATCTTGACATCCTTCAGCG-(DABCYL) |
| BAC16S, F | TGGAGCATGTGGTTTAATTCGA |
| BAC16S, R | TGCGGGACTTAACCCAACA |
| BAC16S, MB | (ROX)-CGCTGGCGAGCTGACGACARCCATGCACCAGCG-(DABCYL) |
| lukF, F | GCCAGTGTTATCCAGAGG |
| lukF, R | CTATCCAGTTGAAGTTGATCC |
| lukF, MB | (FAM)-CGCGAAGAATTTATTGGTGTCCTATCTCGATCGCG-(DABCYL) |
| vanA, F | AATCGGCAAGACAATATGAC |
| vanA, R | ACCTCGCCAACAACTAAC |
| vanA, MB | (ROX)-GGACGTGTGAGGTCGGTTGTGCGGTATACGTCC-(DABCYL) |
a
Molecular beacon (MB) and forward (F) and reverse (R) primer sequences for each of the targets probed for in the blinded panel of bacterial species.
b
Underlined sequences represent stem structure of molecular beacons. DABCYL, 4-(4′-dimethylaminophenylazo)benzoic acid; FAM, fluorescein; HEX, hexachlorofluorescein; ROX, rhodamine; BHQ-2, Black Hole Quencher 2.