Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Escherichia coli)	B-95.ΔA E. coli	Addgene	Bacterial strain #197933	Electrocompetent cells
Recombinant DNA reagent	pDule2-Mj Acd A9 (plasmid)	Addgene	Plasmid #197652	Provided by GCE4Alls
Software, algorithm	TDlifetime_20_Igor_procedures.ipf	This paper	https://github.com/zagotta/TDlifetime_program	Code used for analyzing TCSPC lifetime data for use in tmFRET experiments.