FIG. 5.

hRIPα contains the specific domain for cytoplasmic localization. (A) Schematic diagram of hRIPα, its isoforms, and deletion mutants. Numbers correspond to the amino acid sequence. Note the presence or absence of the indicated region (amino acids 163 to 180) in each construct. (B) hRIPα contains the specific domain (amino acids 164 to 180) required for cytoplasmic retention. The constructs shown in panel A were transiently expressed in HEK293T cells and stained with anti-hRIP antibody (green).