FIG. 6.

hRIPβ transports RPA into the PML nuclear body. HEK293T cells were transfected with a plasmid encoding FLAG-RPA in combination with either blank vector or hRIP constructs. After 24 h, the cells were fixed and stained with anti-hRIP antibody (green) and anti-FLAG antibody (red). (B) Colocalization of RPA with hRIP. HEK293T cells were stained with anti-hRIP antibody (green) and anti-RPA p32 antibody. To remove the background, anti-hRIP antibody was purified as described in Materials and Methods. Bars, 10 μm.