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PhLP1-GFP is localized in the cytosol. An extrachromosomal plasmid expressing a C-terminal fusion of GFP to PhLP1 was expressed in phlp1− cells, which completely restored the aggregation-negative phenotype. PhLP1-GFP appears to be localized in the cytosol in both weakly and strongly expressing cells, without indications for association to membranes or membrane-associated proteins.
